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It is legal for women to be topless in Ontario after a court ruling in 1996.
Protestors held signs that included the slogans "They are boobs not bombs, chill out" and "Nudity isn't sexual."
The three sisters, Tameera, Nadia and Alysha Mohamed, say that they took their shirts off because it was a hot summer day.
However, they allege that a police officer approached them and told them to cover up. But when they challenged this, the officer said he was stopping them for bike safety reasons.
One of the sisters is an award nominated Canadian singer under her stage name Alysha Brilla.
"I had no idea how polarizing the issue would be. I thought people wouldn't be so disturbed by the female breast," she told CBC news.