It looks like you're using an Ad Blocker.
Please white-list or disable AboveTopSecret.com in your ad-blocking tool.
Some features of ATS will be disabled while you continue to use an ad-blocker.
Leap forward for development proteomics
Published: Mar 31, 2014
……Dovichi and his colleagues were able to identify and measure changes in the concentration of nearly 4000 different proteins, reporting their findings in Scientific Reports. As expected, the expression of these proteins changed in step with key events in the physiological development of the X. laevis embryo, with the majority of the proteins involved in biological processes such as DNA replication, transcription and translation, energy generation, protein transport and protein folding. Less expected was the finding that levels of specific proteins did not always correspond to levels of the mRNAs that code for them, confirming that protein expression is controlled via several different mechanisms.
As well as showing how embryonic development normally occurs, this work could also reveal information about congenital birth defects caused by misregulation of gene expression and about the effects of disease on embryo development. ‘It's easy to manipulate the embryos to mimic certain disease states, making Xenopus extremely valuable to biologists,’ says team member Paul Huber.
Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development
While there is a rich literature on transcription dynamics during the development of many organisms, protein data is limited. We used iTRAQ isotopic labeling and mass spectrometry to generate the largest developmental proteomic dataset for any animal. Expression dynamics of nearly 4,000 proteins of Xenopus laevis was generated from fertilized egg to neurula embryo. Expression clusters into groups. The cluster profiles accurately reflect the major events that mark changes in gene expression patterns during early Xenopus development. We observed decline in the expression of ten DNA replication factors after the midblastula transition (MBT), including a marked decline of the licensing factor XCdc6. Ectopic expression of XCdc6 leads to apoptosis; temporal changes in this protein are critical for proper development. Measurement of expression in single embryos provided no evidence for significant protein heterogeneity between embryos at the same stage of development.