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originally posted by: djz3ro
a reply to: madmac5150
I don't care what politicians and the media say, it was the medical professionals I was listening to
originally posted by: IAMTAT
originally posted by: chr0naut
a reply to: madmac5150
Did any of you have a positive PCR test, or where you just sick while it was in the news?
PCR tests are crap for diagnosing China Virus....especially at the scan cycle threshold they've used.
Even Fauci was caught saying so.
In mid-November, none other than he who should not be questioned – Dr. Anthony Fauci – admitted that the PCR Test’s high Ct is misleading:
“What is now sort of evolving into a bit of a standard,” Fauci said, is that “if you get a cycle threshold of 35 or more … the chances of it being replication-confident are minuscule.”
“It’s very frustrating for the patients as well as for the physicians,” he continued, when “somebody comes in, and they repeat their PCR, and it’s like [a] 37 cycle threshold, but you almost never can culture virus from a 37 threshold cycle.”
So, I think if somebody does come in with 37, 38, even 36, you got to say, you know, it’s just dead nucleotides, period.”
So, if anyone raises this discussion as a “conspiracy”, refer them to Dr.Fauci.
principia-scientific.com...
ALL PCR tests are run @ 35-45 CTs.
That means that most...if not all...China Virus infections diagnosed using PCR were FALSE POSITIVES...actually measuring "dead nucleotides".
It means that ANYONE who died of ANYTHING...can...and will be diagnosed as having China Virus...and would've have been falsely added to the CV death toll.
originally posted by: IAMTAT
originally posted by: djz3ro
a reply to: madmac5150
I don't care what politicians and the media say, it was the medical professionals I was listening to
So does THIS GUY come under the heading of "politician"...or "medical professional"?
Listen to him...or no?
weird conspiracy bull is crazy
Seems like as Americans we would come together to
fight this like we’ve came together so many
times in the past to fight adversities.
Wish someone would explain where all the hate is coming from?
originally posted by: SaturnFXMost people who drive drunk don't kill people.
Therefore precautions to not have people drive drunk shouldn't be taken.
originally posted by: chr0naut
Also, each cycle of PCR amplification replicates the genomic sequences present in the sample. I.e, it doubles them every time. If there is no genomic sequence present, it does not make things up. PCR amplification is not like electrical or optical amplification where things become fuzzy the more amplification is applied.
If you google "PCR amplification plot" and have a look at the graphs, you will see that after the exponential phase, there is very little change in accuracy. In fact, it is a case of diminishing returns, where further amplifications will not reveal anything different from previous cycles. If there were more false positives from higher cycle numbers, then the graph would not roll off and become linear as it does. The whole premise that higher amplifications produce greater amounts of error, is a total misunderstanding of what PCR does. Higher amplifications cycles past the exponential stage are simply a waste of time. They won't reveal anything different than previous cycles.
originally posted by: Jimy718
originally posted by: chr0naut
Also, each cycle of PCR amplification replicates the genomic sequences present in the sample. I.e, it doubles them every time. If there is no genomic sequence present, it does not make things up. PCR amplification is not like electrical or optical amplification where things become fuzzy the more amplification is applied.
Well Physics isn’t your strong suit!
In optical amplification, or rather magnification there are limits on how small we can see, so at some point that “property” goes into ‘saturation’, meaning you have reached the physical limit. In electronic amplification we see the same thing, at some point we cannot amplify any more, we have reached ‘saturation’.
In neither case have we reached a state where anything ‘becomes fuzzy’, that is a phenomena that occurs ONLY with digital systems.
If you google "PCR amplification plot" and have a look at the graphs, you will see that after the exponential phase, there is very little change in accuracy. In fact, it is a case of diminishing returns, where further amplifications will not reveal anything different from previous cycles. If there were more false positives from higher cycle numbers, then the graph would not roll off and become linear as it does. The whole premise that higher amplifications produce greater amounts of error, is a total misunderstanding of what PCR does. Higher amplifications cycles past the exponential stage are simply a waste of time. They won't reveal anything different than previous cycles.
This makes the select of Ct to diagnose, and Ct[cutoff] critical; those selections have not been made with the best of care or science, and appear to be more politically motivated.
With this disparity of Ct selection it may be possible to show, statistically, that the majority of covid-19 cases were in fact false positives. A phenomena that occurs with over amplification (this is proven; go read).
ETA: all manufacturers of PCR equipment recommend Ct[cutoff] of 40 or more cycles. The actual Ct for each specimen is recorded by the machine.
originally posted by: chr0naut
originally posted by: Jimy718
originally posted by: chr0naut
Also, each cycle of PCR amplification replicates the genomic sequences present in the sample. I.e, it doubles them every time. If there is no genomic sequence present, it does not make things up. PCR amplification is not like electrical or optical amplification where things become fuzzy the more amplification is applied.
Well Physics isn’t your strong suit!
It is.
LOL
No, with PCR amplification, you are measuring a specific genomic sequence by chemically reproducing it exactly.
It doesn't replicate something other than the sequences in the sample. It only produces exact copies of the sequences.
The more you have of the sequence, the more assurance you have that you are seeing the sequence. However, after about 20 amplification runs, the amount of genomic sequence in the sample cannot increase any more, because the sample become increasingly closer to 100% just genomic sequence, and no support medium. You don't want to run too many cycles because it won't tell you anything more than you already know. Those excess cycles and a waste of time.
That is not true. PCR is not capable of inventing genomes that aren't there. It doesn't get false readings because there are too many amplification cycles. The readings become more assured, up to a point where the result isn't going to change.
This is fairly basic, and omits some of the details, but it will give you a clearer idea: Explainer: How PCR works
originally posted by: Jimy718
originally posted by: chr0naut
originally posted by: Jimy718
originally posted by: chr0naut
Also, each cycle of PCR amplification replicates the genomic sequences present in the sample. I.e, it doubles them every time. If there is no genomic sequence present, it does not make things up. PCR amplification is not like electrical or optical amplification where things become fuzzy the more amplification is applied.
Well Physics isn’t your strong suit!
It is.
LOL
If you say so...
No, with PCR amplification, you are measuring a specific genomic sequence by chemically reproducing it exactly.
It doesn't replicate something other than the sequences in the sample. It only produces exact copies of the sequences.
The more you have of the sequence, the more assurance you have that you are seeing the sequence. However, after about 20 amplification runs, the amount of genomic sequence in the sample cannot increase any more, because the sample become increasingly closer to 100% just genomic sequence, and no support medium. You don't want to run too many cycles because it won't tell you anything more than you already know. Those excess cycles and a waste of time.
Yes, exactly! However, your amplification also amplifies genomic fragments (of the virus), as well as dead virus, at some point these start to be come significant, and may be falsely recognized as a 'live virus'; this is what happens with over amplification (beyond 28 cycles).
That is not true. PCR is not capable of inventing genomes that aren't there. It doesn't get false readings because there are too many amplification cycles. The readings become more assured, up to a point where the result isn't going to change.
This is fairly basic, and omits some of the details, but it will give you a clearer idea: Explainer: How PCR works
As I've said, there are no "new" genomes present, however; genomic fragments, and dead virus are. It is these fragments and dead virus that account for false readings, making the results less reliable after 28 cycles.
ETA: Ya know, until right now I didn't properly appreciate the "n+1" cycles, it didn't make sense. However, mRNA is a strand of RNA which isn't a double stranded helix, just a single strand. So, it must first be make into a double strand (making it DNA) so that it may be amplified...some times, I can be a bit slow, excuse me I'm 74.
originally posted by: chr0naut
Distortion in an analogue signal can occur either through waveform clipping, or through through resonant effects such as feedback (inductive regeneration).
originally posted by: Jimy718
originally posted by: chr0naut
Distortion in an analogue signal can occur either through waveform clipping, or through through resonant effects such as feedback (inductive regeneration).
Well, feedback isn't a resonant effect, but, I guess we can't expect you to know that. Although, in some special cases, resonant feedback might be used, such as in filters...PCR doesn't filter does it? (don't answer, I already know).
And yes 'clipping', perhaps something similar to what might happen to an over amplified sample from someone who has recovered...i.e. dead virus, and not much of it either.
Anyway, what you said was complete "horse"; the electronic world don't quite work that way.
originally posted by: chr0naut
I didn't say all feedback was resonant. I even gave an example of a feedback type that is resonant (inductive regeneration).
And you still don't seem to have understood that PCR amplification is a molecular copying process.
originally posted by: Jimy718
originally posted by: chr0naut
I didn't say all feedback was resonant. I even gave an example of a feedback type that is resonant (inductive regeneration).
The problem is that "inductive regeneration" is not a form of feedback except in automobiles. Inductive Regeneration is what happens when you take your foot off the accelerator pedal in an EV car. It has no other application in electronics that I am aware of.
Oh, I understand it quite well, though you don't quite seem to have come up to speed. Try finding and reading something a wee bit more technical than that simple "how it works", there are plenty of papers to select from on the more technical aspects of PCR...go read.
And you still don't seem to have understood that PCR amplification is a molecular copying process.
originally posted by: chr0naut
In standard radio sets, frequency control can be achieved by a variable LCR circuit where L is an inductance, C is a capacitance and R is a resistance. This allows for a tune-able circuit that uses controlled resonance to select its optimal frequency. So inductive regeneration is used in nearly all analogue electronics, not just in electric vehicles.
Here's some updated stuff you can build for yourself:
PCR on a shoestring: Build Your Own PCR Machine
originally posted by: JAGStorm
a reply to: madmac5150
Starting to see more things like this on social media..hmmmm
vaccinated sicker than non??? You don't say...