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Those detained include senior aides to opposition leader Mir Hossein Mousavi, and a former foreign minister.
Mr Mousavi's nephew, Seyed Ali Mousavi, was among those killed on Sunday.
State media said authorities were doing forensic tests on his and four other bodies, preventing the rapid burials that are usual under Islamic tradition.
The bodies had been "retained in order to complete forensic and police examinations and find more leads on this suspicious incident", the Irna news agency reported.