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The UK has effectively ratified the EU's reform treaty - despite the decision by Irish voters to reject it.
A last-ditch Tory bid to delay the process for four months was defeated by a margin of 93, and peers later gave the EU Amendment Bill a third reading.
Royal Assent is expected within 24 hours, as EU leaders are set to meet in Brussels to discuss the crisis caused by the Irish "No" vote.
During the Lords debate four protesters were removed from the public gallery after voicing their demands for a referendum.
The Tories have issued a St George's Day rallying cry against plans by Brussels to "wipe England off the map" and create a United Europe.
As Gordon Brown hoisted the English national flag over 10 Downing Street to celebrate St George's Day, it was revealed that EU officials had revised a map wiping out the country and the Channel.
The change splits England into three and lumps those parts together with chunks of other countries to create "transnational regions".
With the queen set to approve the so-called EU Amendment Bill by pronouncing the Norman French formula "La Reine le veult" over the document today, the treaty will become British law around 10:00 local time, ahead of the EU meeting this afternoon.
Prime Minister Gordon Brown has ruled out a public vote on the treaty, saying it does not alter the UK constitution. A UK Constitution which is based on Common Law and Acts of Parliament, notoriously never penned down as one physical document, now faces the existence of such a piece.