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Tehran initially said it hoped to resume uranium conversion on Wednesday - the day ultra-conservative President Mahmoud Ahmadinejad was confirmed.
But it has delayed to allow the UN to install necessary equipment to oversee activities at Iran's Isfahan plant.
Tehran's recent move has threatened to derail talks with the European Union.
"We hope to restart work by the beginning of next week when preparations are complete," Mr Rohani told state television. Iran's week begins on Saturday.
"The three European ministers have said that if we restart, this would mean the end of negotiations. This is a threat, this is unacceptable," he said.
"There is no judicial or political logic to send the issue to the Security Council. This would mean that the Europeans have given in to US pressure and they must assume the consequences."
"Once the Isfahan plant restarts, we want to continue the negotiations with the Europeans," he added.